This page provides links to the Interactive Lab Manual for BIOL 1406. Each lab exercise in the manual consists of 3 parts: a Pre-lab (divided into from 1 to 12 sections), the Lab Procedures, and a Post-lab. The Pre-labs are interactive. All Pre-labs contain practice questions with hints and the opportunity for students to check their answers. Many Pre-labs also contain embedded videos and animations covering basic lab techniques or biological concepts that are needed to understand and complete the lab exercise. The Lab Procedures and the Post-labs are non-interactive pdf versions of the printed lab manual.
Please note that the links on this page allow you to access only part of the Interactive Lab Manual for BIOL 1406. If you access the lab manual through Blackboard, there are computer-graded practice quizzes and assessment quizzes for each Pre-lab section. The practice quizzes are designed to provide students with immediate feedback on their mastery of the Pre-lab material while assessment quizzes are designed to provide feedback to instructors. For information on how to access the lab manual through Blackboard, please contact Dr. Sal Tavormina (contact information on the left).
Introduction and Safety Information
1. Introduction
2. Safety Information
3. Safety Video
Exercise 1: How do scientists collect and analyze data?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 2: How do scientists prepare solutions with specific concentrations of solutes?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 3: Understanding pH and buffers; Introduction to the brightfield microscope
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 4: Cell Structure and Diversity
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 5: How are substances transported within cells and across cell membranes?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 6: What factors affect enzyme activity?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 7: How is thin layer chromatography used to separate a mixture of molecules?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 8A: How can α-lactalbumin be separated from the other molecules in milk?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 8B: What is the concentration of protein in your milk fractions?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 8C: How can gel electrophoresis be used to separate the proteins present in your milk fractions?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 8D: How can gel electrophoresis be used to analyze the milk fractions that were collected during your purification of α-lactalbumin?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 9A: Transformation of E. coli and digestion of plasmid DNA with restriction enzymes.
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 9B: Which unknown plasmid sample had no plasmids, which had non-recombinant pUC18 plasmids, and which had recombinant pUC18 plasmids?
1. Pre-lab
2. Lab Procedures
3. Post-lab
Exercise 10: Using Biological Databases on the Internet
1. Pre-lab
2. Lab Procedures
Appendices
A. Keeping a Laboratory Notebook
B. Mean and Standard Deviation Calculations
C. Calibration of Instruments
D. Absorbance Measurements
E. Graphing Data
F. Math Review
Overview
Preparation Before Class
Active Learning During Class
Multimedia Activities
Discussion Questions
Interactive Lab Manual
Dr. Salvatore Tavormina
tav@austincc.edu
RGC Main 342
Phone: 223-3326
https://accmultimedia.austincc.edu/tav